Adeno-associated virus helper activity of adenovirus DNA binding protein
نویسندگان
چکیده
منابع مشابه
The host response to adenovirus, helper-dependent adenovirus, and adeno-associated virus in mouse liver.
Understanding host responses to viral gene therapy vectors is necessary for the development of safe and efficacious in vivo gene transfer agents. We describe the use of high-density spotted complementary DNA microarrays in monitoring the in vivo host transcriptional responses in mouse liver upon administration of either a "first-generation"adenoviral (Ad) vector, a helper-dependent "gutless" ad...
متن کاملProduction of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus.
Recently, efficient and long-term in vivo gene transfer by recombinant adeno-associated virus type 2 (rAAV) vectors has been demonstrated in a variety of tissues. Further improvement in vector titer and purity will expedite this in vivo exploration and provide preclinical information required for use in human gene therapy. In an effort to obtain higher titers, we constructed a novel AAV helper ...
متن کاملFeasibility of generating adeno-associated virus packaging cell lines containing inducible adenovirus helper genes.
The adeno-associated virus (AAV) vector system is based on nonpathogenic and helper-virus-dependent parvoviruses. The vector system offers safe, efficient, and long-term in vivo gene transfer in numerous tissues. Clinical trials using AAV vectors have demonstrated vector safety as well as efficiency. The increasing interest in the use of AAV for clinical studies demands large quantities of vect...
متن کاملAdeno-associated Satellite Virus Interference with the Replication of Its Helper Adenovirus
Adeno-associated satellite virus type 4 interferes with the replication of its helper adenovirus. No interferon-like soluble substance could be detected in satellite-infected cultures and other DNA- and RNA-containing viruses were not inhibited by coinfection with satellite virus under conditions which reduced adenovirus yields by more than 90% in monkey cells. Altering the concentration of ade...
متن کاملGeneration of Helper Plasmids Encoding Mutant Adeno-associated Virus Type 2 Capsid Proteins with Increased Resistance against Proteasomal Degradation
Objective(s): Adeno-associated virus type 2 (AAV2) vectors are widely used for both experimental and clinical gene therapy. A recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. In this study, a fast and simple method is presented to generate AAV2 vector helper plasmids encod...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1982
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.44.2.666-673.1982